Research Paper Volume 16, Issue 3 pp 2438—2456

Figure 1. High level of Mark4 expression was associated with increased risk of vascular calcification. (A) The RT–qPCR showed expression of Marks 1- 4 in HASMCs with high phosphate (n = 4 per group). Error bar represent four independent experiments. ^**Means p < 0.01, ^***p < 0.001 vs. control group. (B) Mark4 mRNA level in serum from patients from CKD with (n = 11) or without VC (n = 10). (C) Correlation between the Mark4 mRNA level and VC scores in patients with CKD (n = 21, the Pearson’s correlation coefficient r value and the p-value are shown). (D) Von Kossa assay and IF staining for Mark4 in radial arteries sections from patients with CKD (n = 4 per group). Scale bars: 50 μm. (E) The bars showing fluorescence intensity of Mark4 in arteries between patients from CKD with or without VC (n = 4 per group). Error bar represent four independent experiments.