Research Paper Volume 16, Issue 3 pp 1983—2004

Figure 1. Penetrance of GV1001 into the brain through the blood-brain barrier. (A, B) We performed magnetic resonance imaging (MRI) to determine whether GV1001 could enter the brain. Ferrocenecarboxylic acid (Fe)-GV1001 (1 mg/kg) was subcutaneously injected into 3xTg-AD (12-month-old) and wild-type (WT) (8-month-old) mice. After 1 h, two-dimensional (2D) axial (Axl) fast field echo (FFE) and 2D coronal (Cor) FFE images were acquired using 3 T MRI. The entire brain was observed in several sections in the dorsoventral and anteroposterior planes using 2D Axl FFE and 2D Cor FFE images. This result shows that GV1001, represented by dark signals, entered the brains of both 3xTg-AD and WT mice (yellow circles in A and B, respectively). (C) Prussian blue staining.