Figure 12. Unfavorable impact of AL049840.4 on HCC in vitro. (A) Validation of knockdown efficiency of AL049840.4 expression in HepG2 cells by qRT-PCR. (B) Cell viability of HepG2 cells after silencing AL049840.4 was detected by CCK-8 assay. (C, D) Cell apoptosis assay revealed that knockdown of AL049840.4 promoted apoptosis in HepG2 cells. (E, F) Transwell assay showed a statistically significant decrease in the HepG2 cells migration after AL049840.4 knockdown.