Research Paper Volume 16, Issue 3 pp 2362—2384
Prepared Radix Polygoni Multiflori and emodin alleviate lipid droplet accumulation in nonalcoholic fatty liver disease through MAPK signaling pathway inhibition
Figure 7. Emodin mediates the AMPK signaling pathway in HepG2 cells. (A) IF staining of SREBP-1. (B) Florescence intensity analysis of SREBP-1, ×200. (C) Representative Western blot of SREBP-1, ACC1, FASN, and ACLY. (D–G) Western blot analysis. (H–K) Relative mRNA levels of SREBP-1, ACC1, FASN, and ACLY (ratio: measured mRNA/GAPDH mRNA). (L–N) Nile red and Oil red O staining and analysis. *indicates comparison with NC, P < 0.05. A indicates a comparison with FFA Medium, P < 0.05. The white line indicates 50 μm.