Research Paper Volume 16, Issue 3 pp 2362—2384
Prepared Radix Polygoni Multiflori and emodin alleviate lipid droplet accumulation in nonalcoholic fatty liver disease through MAPK signaling pathway inhibition
Figure 5. Emodin mediates the MAPK signaling pathway in HepG2 cells. (A) IF staining of P38, phosphorylated P38, ERK1/2 and phosphorylated ERK1/2 (200×) in HepG2 cells. (B–E) Florescence intensity analysis of P38, phosphorylated P38, ERK1/2 and phosphorylated ERK1/2. (F) Representative Western blot of P38, phosphorylated P38, ERK1/2 and phosphorylated ERK1/2. (G–J) Western blot analysis. *indicates a comparison with NC, P < 0.05. The white line indicates 50 μm.