Research Paper Volume 16, Issue 2 pp 1938—1951

Figure 2. LncMEG3 inhibits PCa cells proliferation and promotes apoptosis in vitro. (A, B) qRT-PCR assays were applied to analyse the expression level of lncMEG3 after transfection by lentivirus or siRNA in PC3 or LNCaP cells. (C) Western blot analysis revealed that the levels of cell apoptosis-related proteins (Bcl-2, cleaved-caspase3) and cell cycle-associated protein (Cyclin D1) were significantly altered after lncMEG3 regulated. (D) Immunofluorescence images of Ki-67 expression in LNCaP and PC3 cells with lncMEG3 downregulation or upregulation. Scale bar, 20μm. (E) Colony forming assay performed in LNCaP and PC3 cells to evaluate cell proliferation ability. (F, G) Cell viability of LNCaP and PC3 cells after knocking down or overexpressing lncMEG3 was determined using CCK8 assays. EdU assays also implicated the same results. (H, I) Cell cycle distribution and apoptosis were analysed by flow cytometry in LNCaP and PC3 cells, * P < 0.05.