Research Paper Volume 16, Issue 2 pp 1925—1937

Figure 5. MCAO model verifies the role of MDM2 in regulating Melatonin to improve brain injury. (A) The CCK-8 assay indicates that the protective effects of Melatonin on HT-22 cell viability were reversed upon MDM2 knockdown. (B, C) The EdU and TUNEL assay demonstrates that MDM2 knockdown eliminates the effects of Melatonin in improving HT-22 cell proliferation and apoptosis. (D) DCFH-DA staining shows that MDM2 knockdown hinders the effects of Melatonin in reducing ROS levels. (E) Detection of GSH reveals that MDM2 knockdown nullifies the effects of Melatonin in increasing GSH levels. (F) QRT-PCR and WB analysis illustrates that MDM2 knockdown reverses the inhibitory effects of Melatonin on ACSL4 expression. Data are presented as mean ± SD. ^*P < 0.05 vs. Con group; ^#P < 0.05 vs. si-NC group; ^&P < 0.05 vs. MT-H group.