Research Paper Volume 16, Issue 2 pp 1897—1910

Figure 7. IMP3-depleted inhibits OSCC cell proliferation. (A) Establishment of IMP3-depleted SCC-25 cells. The expression of IMP3 was analyzed with qRT-PCR and Western blot. Relative expression levels were calculated using the image J software (n = 3). (B) Stable IMP3-depleted SCC-25 cells and control cells were subcutaneously injected into nude mice. A representative image showing tumors from mice transplanted with SCC-25 cells with or without IMP3 inhibition. (C) The tumor growth and the tumor weights from each group were statistically analyzed (n=6). (D) qRT-PCR and Western blot analysis showed that IMP3 and E2F5 levels were increased in the tissues compared with the control tissues. (E) IHC staining of IMP3 (up) and E2F5 (down) in tumor tissues from mice implanted with SCC-25-LV-si-IMP3 or SCC-25-LV-si-Ctrl cells. Magnification ×40. Scale bar, 50 μm. Relative expression levels were calculated using the image J software (n = 6). P ≤ 0.05 was considered to be statistically significant, *P < 0.05, **P < 0.01, ***P < 0.001.