Research Paper Volume 16, Issue 2 pp 1897—1910

Figure 3. IMP3 positively regulated E2F5. (A) TCGA analysis showed that E2F5 mRNA levels were increased in the OSCC tissues (n=352) compared with the normal tissues (n=32). (B) Pearson correlation analysis of IMP3 and E2F5 expression in OSCC showed that IMP3 is positively correlated with E2F5. (C, D) qRT-PCR and Western blot assay showed that E2F5 was upregulated in OSCC cell lines (SCC-25 and SCC-4) in comparison with HOK cell line. (E) Representative images of IHC staining of E2F5 protein in OSCC tissues (n=40). Magnification ×40. The scale bar indicates 50 μm. (F) Quantification of E2F5 transcripts in SCC-25 and SCC-4 after transfection with siIMP3. Relative expression levels were calculated using the image J software (n = 3). P ≤ 0.05 was considered to be statistically significant, **P < 0.01, ***P < 0.001.