Research Paper Volume 16, Issue 2 pp 1897—1910

Figure 1. Overexpression of IMP3 in human OSCC tissues and cell lines. (A) TCGA analysis showed that IMP3 mRNA levels were increased in the OSCC tissues (n=352) compared with the normal tissues (n=32). (B) Kaplan-Meier analysis was performed on OSCC patient survival with the GEPIA database according to high or low expression of IMP3. (C) Methylation sequencing results showed that the high expression of IMP3 in oral squamous cell carcinoma was probably related to CpG island methylation modification. Expression level of IMP3 mRNA (D) and protein (E) in OSCC cell lines (SCC-25 and SCC-4) in comparison with HOK cell line. (F) The IHC score of IMP3 was markedly higher in the OSCC tissues than in the adjacent normal tissues (n=40). Magnification ×40. The scale bar indicates 50 μm. Relative expression levels were calculated using the image J software (n = 3). P≤ 0.05 was considered to be statistically significant, **P <0.01, ***P< 0.001.