Research Paper Volume 16, Issue 2 pp 1796—1807
Circ0060467 sponges miR-6805 to promote hepatocellular carcinoma progression through regulating AIFM2 and GPX4 expression
Figure 4. Circ0060467 regulates the expression of GPX4 and AIFM in HCC. (A) Predicted direct binding sites of hsa-miR-6085 (miR-6085) within the apoptosis inducing factor mitochondria associated 2 (AIFM2) and glutathione peroxidase 4 (GPX4) sequences. (B) Expression level of GPX4 and AIFM2 in the LO2 cell line and hepatocellular carcinoma cell lines. (C) Expression levels of GPX4 and AIFM2 after transfection with miR-6085 mimics. (D) RNA immunoprecipitation (RIP) assay detected the relative enrichment of circ0060467, GPX4, AIFM2, and miR-6085 in the anti-Argonaute 2 (Ago2) fraction (left); relative enrichment was detected by an Ago2-RIP assay (right).