Research Paper Volume 16, Issue 2 pp 1796—1807
Circ0060467 sponges miR-6805 to promote hepatocellular carcinoma progression through regulating AIFM2 and GPX4 expression
Figure 3. Circ0060467 functions as a sponge for miR-6085 in hepatocellular carcinoma (HCC) cells. (A) Predicted binding sites of miR-6085 within the circ0060467 sequence. (B) Expression level of miR-6085 in the LO2 cell line and HCC cell lines. (C) Relative luciferase activity of 97H and 7402 cells co-transfected with the miR-6085 mimics and the luciferase reporter vector containing the wild-type or mutant circ0060467 sequence. (D) miR-6085 expression in 7402 and 97h cell lines with knockdown of circ-0060467. (E) MS2-based RNA immunoprecipitation assay in 7402 cell transfected with MS2bs-circ0060467, MS2bs- circ0060467-mt, or MS2bs-Rluc. **p < 0.01.