Research Paper Volume 16, Issue 2 pp 1767—1780
Tremella fuciformis polysaccharides induce ferroptosis in Epstein–Barr virus-associated gastric cancer by inactivating NRF2/HO-1 signaling
Figure 6. TFP-induced ferroptosis was mediated via NRF2 in gastric cancer cells. EBV-infected AGS and MKN45 cells were preincubated with TFP for 2 h. After that, Ad-NRF2 and Ad-NC were transfected into EBV-infected AGS and MKN45 cells for 24 h. Transfection with ad-NRF2 abolished TFP-induced suppression of HO-1, GPX4 and xCT in AGS (A) and MKN45 (B) cells. Overexpression of NRF2 reduced intracellular Fe2+ (C), SOD (D) and MDA (E) levels, even in the presence of TFP, in EBV-infected AGS and MKN45 cells. (F) Elevated NRF2 expression increased the level of GSH, and TFP pretreatment could not affect such effects in EBV-transfected GC cells. *p<0.05, **p<0.01, ***p<0.001 vs. con.