Research Paper Volume 16, Issue 2 pp 1733—1749
Identification and validation of methylation-CpG prognostic signature for prognosis of hepatocellular carcinoma
Figure 6. Regulation of BMPER expression by promoter methylation. (A) Methylation level of cg17561435 in different HPSM subgroups. (B) Negative correlation between BMPER mRNA expression and cg17561453 methylation levels. (C) Prediction of CpG islands in the BMPER promoter using the MethPrimer website, and detection of BMPER promoter methylation status using bisulfite sequencing PCR (BSP). (D) Quantification of BMPER mRNA expression levels after 5-aza-dC treatment using qPCR (***p < 0.01). (E) Detection of BMPER protein expression levels after 5-aza-dC treatment using western blotting.