Research Paper Volume 16, Issue 2 pp 1663—1684
Usenamine A triggers NLRP3/caspase-1/GSDMD-mediated pyroptosis in lung adenocarcinoma by targeting the DDX3X/SQSTM1 axis
Figure 5. DDX3X interacts with SQSTM1 in LUAD cells. (A) The interaction between DDX3X and SQSTM1 was identified by co-immunoprecipitation. (B) Immunofluorescence detected the subcellular co-location of DDX3X and SQSTM1. Scale bar = 25 μm. (C, D) Relative mRNA and protein expression of DDX3X and SQSTM1 in H1299 and A549 cells were measured by RT-qPCR and western blotting, respectively. *p < 0.05 and **p < 0.01 vs. sh-NC; $$p < 0.01 vs. oe-NC. DDX3X, DEAD-Box helicase 3 X-Linked; SQSTMI1, sequestosome 1; LUAD, lung adenocarcinoma; RT-qPCR, reverse transcription-quantitative PCR.