Research Paper Volume 16, Issue 2 pp 1581—1604
Identification of a basement membrane gene signature for predicting prognosis and estimating the tumor immune microenvironment in prostate cancer
Figure 10. Inhibition of THBS2 affects the proliferation and migration of PCa cells in vitro. (A) The expression of THBS2 in TCGA PCa tumor tissues (n = 492) and the normal (n = 52). (B) Protein expression of THBS2 in the normal prostate epithelial cell line (RWPE-1) and PCa cell lines (C4-2B, DU145, C4-2 and PC-3). (C) Western blotting showing THBS2 siRNA knockdown compared to control siRNA treatment. (D) CCK-8 assay showed that THBS2 knockdown inhibited C4-2 and PC-3 cell proliferation. (E) The colony formation assay showed that THBS2 knockdown inhibited C4-2 and PC-3 colony formation. The graph on the right shows the colony numbers from 3 independent experiments. (F) Transwell assays showed that THBS2 knockdown inhibited C4-2 and PC-3 cell migration (Scale bar, 50 μm). The graph on the right shows the migrated cells from 3 independent experiments. All data are presented as the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001.