Research Paper Volume 16, Issue 2 pp 1352—1373

Figure 1. H/R enhanced PMVEC injury via autophagy. (A) Western blot showing the expression of LC3-I/LC3-II and Beclin-1 under treatment of 3-MA, normoxia, hypoxia or H/R. (B) The mRFP-GFP-LC3 dual fluorescence system was used to trace autophagy formation under treatment of 3-MA, normoxia, hypoxia or H/R. (C) CCK-8 assay showing changes in proliferation of cells treated with 3-MA or under normoxia, hypoxia or H/R. (D) Transwell assay showing PMVEC migration (magnification, 200 ×; scale bar = 50 μm) under treatment of 3-MA, normoxia, hypoxia or H/R. (E) Flow cytometry showing apoptosis under treatment of 3-MA, normoxia, hypoxia or H/R. (F) Western blot showing Bcl-2 and Bax, cleaved caspase-3 and caspase-3 expression under treatment of 3-MA, normoxia, hypoxia or H/R. Measurement data are expressed as mean ± standard deviation. Differences between 2 groups of data were compared using an unpaired t-test. Two-way ANOVA was used for data comparison at different time points. * p < 0.05. The cell experiments were repeated three times.