Research Paper Volume 16, Issue 2 pp 1336—1351

Figure 3. The difference in diversity of the gut microbiota between mice in the osteosarcoma model group and mice in the control group. (A) Heatmap of genus-level abundance in each sample. The cluster tree on the left represents species clusters and the upper cluster tree represents sample clustering. Red indicates higher relative abundances of the genus, and blue indicates lower relative abundances of the genus. (B) Differential genus abundance Top10 boxplot. Different colors represent different groups of samples, and the ordinate represents the log-transformed value of the relative abundance of genera. (C) PCoA 3D diagram. Each sample is represented by one point in the Figure, and samples from the same group are of the same color. The closer the samples in the same group are, with a clear distance from other groups, indicates that the grouping effect is good. (D) Differential species annotation cladogram from LEfSe analysis. Nodes with different colors, respectively, indicate significantly different species with relatively high abundance in the different groups. Yellow nodes indicate species that were not significantly different between the two groups. The node diameter is proportional to the relative abundance. (E) Differential species score chart. Groups are represented by different colors. Genera in group A with a relatively high abundance are displayed in red bars, and green represents group B. Abbreviations: PCoA: principal coordinate analysis; LEfSe: linear discriminant analysis coupled with effect size measurements.