Research Paper Volume 15, Issue 24 pp 15578—15598
Development of a prognostic model for glioblastoma multiforme based on the expression levels of efferocytosis-related genes
Figure 9. In vitro validation of PDIA4 in GBM efferocytosis. (A, B) PDIA4 knockdown efficacy was detected by Western blotting. (C, D) Exposure of phosphatidylserine on apoptotic cell surface was evaluated by Annexin V level. (E, F) Efferocytosis was indicated by proportion of CD11b+CFSE+ cells. (G, H) Quantification of the expression level of macrophages-associated marker (CD206) by flow cytometry. (I, J) Measurement of IL-10 and TGF-β levels in cocultured medium of efferocytosis assays. Data are presented as mean ± SD.