Research Paper Volume 15, Issue 24 pp 15578—15598
Development of a prognostic model for glioblastoma multiforme based on the expression levels of efferocytosis-related genes
Figure 2. Efferocytosis-related differentially expressed genes (ERDEGs) were primarily involved in immune responses mediated by cytokines. (A) Volcano plot of DEGs in glioblastoma multiforme (GBM). (B) Heat map of top 10 upregulated and top 10 downregulated genes in GBM. (C) The Hclust function indicated that all samples in the training dataset were well clustered. (D) The soft-threshold power of 10 was selected to calculate adjacencies based on R2 > 0.85. (E) 12 modules were identified by dynamic tree cutting approach. (F) MEyellow and MEtan, which were mainly related to efferocytosis scores were selected, and 1,189 genes were considered as hub genes. (G) 982 ERDEGs were identified by crossing 1,189 hub gens with 7,487 DEGs found between GBM and normal samples. (H) Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of ERDEGs. (I) Gene Ontology (GO) enrichment analysis of ERDEGs.