Figure 7. The microglia profiles in different regions of the brain were evaluated with the immunofluorescent (IBA-1)-labeled staining. (A) Microglia in CA1 were shown with IBA-1 antibody staining. (B) density, (C) body area and (D) branch number of IBA-1 positive cells were analyzed. (E) Microglia in CA3 were shown with IBA-1 antibody staining. (F) density, (G) body area and (H) branch number of IBA-1 positive cells were analyzed. (I) Microglia in DG were shown with IBA-1 antibody staining. (J) density, (K) body area and (L) branch number of IBA-1 positive cells were analyzed. (M) Microglia in the cortex were shown with IBA-1 antibody staining. (N) density, (O) body area and (P) branch number of IBA-1 positive cells were analyzed. Scale bar = 25μm. The scale bar in magnified image = 5μm. [(Con group, n=3), (2VO group, n= 3)].