Figure 2. WB and RT-qPCR were used to detect the level of BRCA1 in BRCA. (A) WB and RT-qPCR (B) results showed that the expression of BRCA1 in breast cancer cells was higher than that in normal breast epithelial cells. (C) WB and RT-qPCR (D) results revealed that the level of BRCA1 in breast cancer tissues was higher than that in paracancerous tissues. The experiments were repeated 3 times. Data are shown as means ± SD. P-values were calculated with two-tailed unpaired Student’s t-test, *p < 0.05, **p < 0.01, ***p < 0.001.