Research Paper Volume 15, Issue 24 pp 15382—15401

Figure 4. Identification of senescence and fibrosis markers in an aging cell model, fibrotic cell model, and fibrotic mouse model. Aging cell models were established in MRC-5 cells using 10 μM etoposide, and 5 ng/mL TGFβ1 was used to establish a fibrotic cell model in MRC-5 cell. The BLM model group was sprayed with 5 mg/kg BLM through the trachea by using a Penn–Century MicroSprayer. (A) The expression levels of collagen III, collagen I, vimentin, α-SMA, FAP1, S100A4, P16, MMP9, STAT1, P62, TGFβ1, and FTH1 increased in the etoposide and TGFβ1 groups compared with those in normal mice. (B) SA-β-gal-positive cells increased in the TGFβ1-stimulated group, but the etoposide group had more β-gal-positive cells than the normal and TGFβ1 groups. SA-β-gal-positive cells are blue. (C) Double immunofluorescence staining showed that S100A4 and P16 were significantly highly expressed in the etoposide and TGFβ1 groups, but S100A4 in the TGFβ1 group was higher than that in the etoposide group, and P16 in the etoposide group was higher than that in the TGFβ1 group. (D) The expression levels of collagen III, collagen I, vimentin, α-SMA, FAP1, S100A4, P16, MMP9, STAT1, P62, TGFβ1, and FTH1 in lung tissue of aged and fibrotic mice were significantly higher than that of the sham group.