Research Paper Volume 15, Issue 24 pp 15382—15401

Figure 3. Impact of aging on mouse lung proteomics. (A) Hierarchical clustering of protein analysis. Blue to red indicate low-to-high expression levels. (B) KEGG pathway analysis exhibited that the screened differentially expressed proteins were enriched in pathways including ECM−receptor interaction, ferroptosis, phagosome, protein digestion and absorption, NF-kappa B signaling pathway, PPAR signaling pathway, and cell adhesion molecules. (C) Differentially expressed proteins related to ferroptosis, autophagy, mitochondria, and fibrosis were screened. (D) Western blot assay identified that the expression levels of FTH1, TGFβ1, P62, STAT1, MMP9 increased in aging mice compared with those in young mice. The in vitro result was consistent with the in vivo one.