Figure 2. PV interneurons in the spinal cord of ALS mice with different age by immunofluorescence. (A) The yellow boxes represent the typical positive cells (enlarged approximate twice on the original position). The typical PV positive cells are enlarged approximate 4 times and shown on the bottom right panel. Scale bar = 100 μM. PV interneurons in the spinal cord of ALS mice with different age by RT-qPCR (B) and western blot (C). ***P < 0.001 vs. control at 90~100 days. ###P < 0.001 vs. control at 130~140 days. ##P < 0.01 vs. control at 130~140 days. In all experiments, half male and half female mice were used in each group (n = 6).