Macrophage-specific deletion of Notch-1 induced M2 anti-inflammatory effect in atherosclerosis via activation of the PI3K-oxidative stress axis

Mingming Zhang; Xiangyong Yue; Xueping Zhao; Yonggang Lu; Hongtao Liu; Zhe Zhang; Huan Ma; Xing Wang; Hanying Xing

doi:doi:10.18632/aging.205342

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Research Paper Volume 15, Issue 24 pp 15196—15212

Macrophage-specific deletion of Notch-1 induced M2 anti-inflammatory effect in atherosclerosis via activation of the PI3K-oxidative stress axis

Figure 2. Specific deletion of Notch-1 in macrophage repressed AS. (A) Oil red O staining in thoracic aortas and quantitative analysis revealed that Notch-1^MAC-KO significantly decreased the atherosclerotic plaques compared with Notch-1^WT. ^**P < 0.01: ApoE^−/−/Notch-1^MAC-KO vs. ApoE^−/−/Notch-1^WT. (B) Movat, α-SMA, CD68 and Sirius red staining and quantitative analysis revealed that Notch-1^MAC-KO could decrease plaque cellularity (Movat staining) and macrophage infiltration (CD 68) compared with Notch-1^WT. However, there was no significant difference in smooth muscle cell content (α-SMA staining) and fibrotic lesions (Sirius red staining) between the two groups. ^**P < 0.01: ApoE^−/−/Notch-1^MAC-KO vs. ApoE^−/−/Notch-1^WT.