Research Paper Volume 15, Issue 24 pp 15161—15182
Reveal the correlation between hub hypoxia/immune-related genes and immunity and diagnosis, and the effect of SAP30 on cell apoptosis, ROS and MDA production in cerebral ischemic stroke
Figure 10. The relative content of SAP30, ROS and MDA and the apoptosis rate in HMC3-si-NC-OGD/R group and HMC3-si-SAP30-OGD/R group. (A) Real time-PCR was used to detect the expression of SAP30 to screen out effective interference targets in HMC3 cell; (B) The relative expression level of SAP30 in HMC3-si-NC-OGD/R group and HMC3-si-SAP30-OGD/R group; (C) Fluorescence value of ROS in HMC3-si-NC-OGD/R group and HMC3-si-SAP30-OGD/R group; (D) Content of MDA in HMC3-si-NC-OGD/R group and HMC3-si-SAP30-OGD/R group; (E) Apoptosis rate in HMC3-si-NC-OGD/R group was detected by flow cytometry; (F) Apoptosis rate in HMC3-si-SAP30-OGD/R group was detected by flow cytometry; (G) Histogram of apoptosis rate in HMC3-si-NC-OGD/R group and HMC3-si-SAP30-OGD/R group. *P < 0.05, **P < 0.01, ***P <0.001, ****P < 0.0001. Q1-2 and Q1-4 quadrants represent late apoptotic cells and early apoptotic cells, respectively.