Research Paper Volume 15, Issue 24 pp 15064—15083
TMX family genes and their association with prognosis, immune infiltration, and chemotherapy in human pan-cancer
Figure 12. TMX2 promotes epithelial-mesenchymal transition (EMT) in liver cancer cells. Verification of sh-TMX2 (A). Transfection analysis of TMX2 (B). Microscopic observations were recorded at 0, 12, and 24 h after scratching the surface of a confluent layer of the indicated HepG2 and Huh-7 cells (C). Quantitative analysis of wound healing percentage in HepG2 cells (D). Quantitative analysis of wound healing percentage in Huh-7 cells (E). The effects of TMX2 on cell migration and invasion were examined by transwell assays in HepG2 cells (F). Quantitative analysis of cell migration in HepG2 cells (G). Quantitative analysis of cell invasion in HepG-2 cells (H). Western blot analysis of EMT marker expression in TMX2-overexpressing HepG2 and Huh-7 cells (I). Western blot analysis of EMT marker expression in TMX2-deficient HepG2 and Huh-7 cells (J). *p < 0.05, **p < 0.01, ***p < 0.001.