Research Paper Volume 15, Issue 24 pp 14945—14956

Figure 4. Effect of an impaired glymphatic system on Angiopep-2 efflux from the brain. (A) Seven days after bilateral DCLN ligation, 5 μl of FITC-Angiopep-2 (0.05 mg/μl, AP -5.0, ML 2.0, DV 3.0) was stereotaxically injected (AP, -5.0 mm; ML, −2.0 mm; DV, +3.0 mm) into the brain parenchyma. (B) Representative brain sections of the two groups (DCLN and SHAM; green, FITC-Angiopep-2; blue, DAPI, 2000 μm). (C, D) Confocal microscope imaging of representative brain sections (DCLN and SHAM; green, FITC-Angiopep-2; blue, DAPI, 50 μm). (E) Quantification of postinjection FITC-Angiopep-2 fluorescent counts in the brain tissue of the two groups. Significance level: **P < 0.05.