Research Paper Volume 15, Issue 24 pp 14915—14929
hsa_circ_0002980 prevents proliferation, migration, invasion, and epithelial-mesenchymal transition of liver cancer cells through microRNA-1303/cell adhesion molecule 2 axis
Figure 2. Overexpression of circ_0002980 memorably suppressed the proliferation of LC cells. (A) HepG2 and Huh7 cells were overexpressed by circ_0002980 through transfection, and the overexpression effect of circ_0002980 was verified with RT-qPCR. (B) Clone formation assay was applied to evaluate the change of clone-forming ability in circ_0002980-overexpressed HepG2 and Huh7 cells. (C) Quantitative analysis of relative colony number. (D) After circ_0002980 overexpression, cell proliferative potential was monitored using flow cytometer with Edu dye. (E) Edu positive cells were quantified. **P < 0.01; ***P < 0.001.