Research Paper Volume 15, Issue 24 pp 14900—14914

Figure 3. Acetylshikonin increased the membrane permeability of NSCLC cells and the proportion of necrotic NSCLC cells. (A, B) Flow cytometry results for Annexin V/PI showing the incidence of cell death among H1299 and A549 cells following treatment with acetylshikonin (0.5–10 μM) for 24 h (n = 4). (C) Phase microscope images of NSCLC cells following incubation with acetylshikonin (0.5–10 μM) for 24 h. Red arrows indicate swollen blebs. Scale bar = 50 μm. (D, E) PI staining results of H1299 and A549 cells following treatment with acetylshikonin (0.5–10 μM) for 4 h. Scale bar = 200 μm. Untreated cells were used as controls. Results are shown as means ± SD. ^*p < 0.05 compared to untreated control.