Research Paper Volume 15, Issue 24 pp 14900—14914

Figure 1. Acetylshikonin decreased cancer cell viability and induced chromatin condensation and nuclear debris formation. (A) Chemical structure of acetylshikonin. (B) CCK-8 assay results of MRC-5 cells and H1299 and A549 cells following incubation with acetylshikonin (0.5–10 μM) for 24 h (n = 4). (C, D) Fluorescence microscope images showing DAPI staining results and cell morphology of H1299 and A549 cells following treatment with acetylshikonin (0.5–10 μM). Red arrows indicated nuclear debris. Scale bar = 50 μm. MRC-5 cells and untreated cells were used as controls. Results are shown as means ± SD. ^*p < 0.05 compared to untreated control.