Research Paper Volume 15, Issue 24 pp 14791—14802
IGFBPL1 inhibits macrophage lipid accumulation by enhancing the activation of IGR1R/LXRα/ABCG1 pathway
Figure 4. IGF-1R is critical for the anti-lipid accumulation effect of IGFBPL1 in macrophages. (A) Cell viability of THP-1 macrophages treated with PPP was detected by CCK-8. (B) Relative mRNA level of IGFBPL1, LXR and ABCG1 in THP-1 macrophages. (C) Representative images of oil red O staining. (D) WB was used to detect the protein level of LXR, ABCG1 and IGFBPL1 (n=3). (E) Quantitative statistics on WB results by ImageJ (n=3). PA: palmitic acid, PPP: picropodophyllin. PA + ov-IGFBPL1 + PPP, THP-1 was transfected for 24 h and then incubated with PA (100 μM) and PPP (1 μM) for 48 h. P<0.05 represents a significant statistical difference; Scale indicates 25 μm.