Research Paper Volume 15, Issue 24 pp 14791—14802

Figure 3. LXR meditated the regulation effect of IGFBPL1 on ABCG1 expression and macrophage lipid accumulation. (A) Relative mRNA level of LXR (n=3). (B) WB was used to detect the protein level of LXR. (C) Quantitative statistics on WB results by ImageJ (n=3). (D) CCK-8 was used to detect the cell viability (n=3). (E) Relative mRNA level of IGFBPL1, IGBPL1, LXR and ABCG1 (n=3). (F) Representative images of oil red O staining. (G) WB was used to detect the protein level of IGFBPL1, LXR and ABCG1. (H) Quantitative statistics on WB results by ImageJ (n=3). PA: palmitic acid, SR9238: LXRα antagonist. PA + ov-IGFBPL1 + SR9238, T THP-1 was transfected for 24 h and then incubated with PA (100 μM) and SR9238 (1 μM) for 48 h. P<0.05 represents a significant statistical difference; Scale indicates 25 μm.