Figure 4.miR-429 increases TNBC cell proliferation, migration, and invasion through degradation of DLC1. (A) Protein levels of DLC1 relative to GADPH in cell assessed by Western Blot after transfection with indicated vectors, i.e., siRNA, miRNA, or inhibitor; (B) The cell proliferation was assessed by CCK-8 after transfection (0h, 24h, 48h,72h); (C) Wound healing assays were used to detect migration of cells after transfection (magnification, × 100). (D) The cell invasion was determined by transwell after transfection (magnification, × 200). All figures were 50 μm of scale bar. (* p < 0.05, ** p < 0.01, and *** p < 0.001).
