Figure 11. Validation of levels of gene expression. In pancreatic tumor tissues, the expression levels of LDHA (A), IL1R2 (B), and TM4SF1 (C) were considerably greater than in healthy tissues. Immunohistochemical examination indicated that the expression levels of LDHA (D) and IL1R2 (E) were considerably elevated in pancreatic tumor tissues compared to healthy tissues, TM4SF1 (F) was moderately expressed in both pancreatic tumor tissues compared to healthy tissues.