Research Paper Volume 15, Issue 18 pp 9572—9589

Figure 1. Cellular senescence occurs in BLM-induced lung fibrosis mice model. (A) HE and Masson staining reveal representative pictures of different lung tissue sections after 21 days of BLM treatment. Scale bar, 100 μm. (B–F) Western blotting using anti-E-cadherin, anti-collagen III, anti-p21, anti-p16, and anti-β-actin antibodies in mice treated with BLM or saline. The grayscale evaluations of the bands were adjusted to be equal to β-actin. Data were presented as the mean ± SD. ^*P < 0.05 compared to control (Unpaired t-test). ^**P < 0.01 compared to control (Unpaired t-test). ^***P < 0.001 compared to the control (Unpaired t-test). (G) SA-β-gal staining reveals representative images of different lung tissue sections. Scale bar, 100 μm. (H–K) The protein levels of collagen III and E-cadherin in different lung tissues were measured via immunofluorescence technique. DAPI was used to counterstain the nuclei. Scale bar 100 μm. Data were presented as the mean ± SD. ^**P < 0.01 compared to control (Unpaired t-test). ^***P < 0.001 compared to the control (Unpaired t-test). (L–O) Representative immunofluorescence shows colocalization of p21^WAF1 or p16^ink4a (Red) and SPC (Green) in lung tissue. DAPI was used to counterstain the nuclei. Scale bar, 50 μm. Data were presented as the mean ± SD. ^**P < 0.01 compared to control (Unpaired t-test). ^***P < 0.001 compared to the control (Unpaired t-test). (P) High performance liquid chromatography shows FUT8 activity in mice. Data are the percentage of the area of P divided by the area of S+P and presented as the mean ± SD. P is the fucosylation product, and S is the peptide substrate. ^*P < 0.05 compared to the control (Unpaired t-test).