Research Paper Volume 15, Issue 24 pp 14607—14616
The protective effects of Phoenixin-20 in tumor necrosis factor α (TNF-α)-induced cell senescence of rheumatoid arthritis fibroblast-like synoviocytes (FLS)
Figure 8. Overexpression of STAT6 abolished the protective effect of Phoenixin-20 on TNF-α-induced cellular senescence in RA-FLSs. Cells were transduced with lentiviral STAT6, followed by stimulation with TNF-α (10 ng/mL) and Phoenixin-20 (20 nM) for 7 days. (A) Western blot analysis revealed successful overexpression of STAT6; (B) mRNA of p53 and p21. (C) Cellular senescence was assayed using SA-β-gal staining (※P < 0.01 vs. vehicle group; ##P < 0.01 vs. TNF-α group, *P < 0.05 vs. PNX-20 group, N = 5–6).