Research Paper Volume 15, Issue 18 pp 9438—9452
Astragalus polysaccharide inhibits the development of urothelial carcinoma by activating AMPK signaling to induce BENC1-xCT complex formation
Figure 4. APS inhibited the activity of xCT and reduced the expression of GPX4. (A) APS significantly reduced the activity of glutathione peroxidase in RT4 and T24 cells. (B) The Western blot results revealed that APS did not change the expression of xCT in RT4 and T24 cells but reduced the protein level of GPX4. (C) The IF results showed that APS did not change the fluorescence level of xCT but significantly inhibited the fluorescence expression of GPX4 (Bar represents 10 μm, 20 × magnification). (D) APS reduced the activity of xCT in RT4 and T24 cells. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Con.