Research Paper Volume 15, Issue 16 pp 8501—8517

Figure 5. Effects of XBJ on apoptosis-related proteins and TLR4/NF-κB/IKKα signaling pathway. (A) Representative images of western blotting for TLR4, p-NF-κB, NF-κB, p-IKKα, Bax, Bcl-2, Bcl-xl, Cleaved-Caspase 3, Cleaved-Caspase 9 and Cleaved-PARP in vivo (GAPDH was used as the internal reference protein). (B–J) Relative intensity of TLR4 (B), p-NF-κB/NF-κB (C), p-IKKα (D), Bax (E), Bcl-2 (F), Bcl-xl (G), Cleaved-Caspase 3 (H), Cleaved-Caspase 9 (I) and Cleaved-PARP (J) was analyzed by western blotting. Data are expressed as mean ± SD (n=3/group), # indicates p<0.05 (vs. Sham group), * indicates p<0.05 (vs. CLP group). (K) Representative images show apoptosis of heart tissue was detected with TUNEL staining.