Research Paper Volume 15, Issue 16 pp 8367—8383

Figure 4. circFAM169A promotes CRC angiogenesis and metastasis in vitro and in vivo. (A) The expression of circFAM169A in FHC, HT29, SW480, HCT116, HCT8, LOVO, and RKO cells determined using qRT–PCR. All data are presented as means ± SD (n = 3 independent experiments). ^*p ≤ 0.05, ^**p ≤ 0.01, ^***p ≤ 0.001, and ^****p ≤ 0.0001. (B, C) Construction of stable inducible circFAM169A-knockdown or -overexpressed CRC cell lines. All data are presented as means ± SD (n = 3 independent experiments). ^***p ≤ 0.001 and ^****p ≤ 0.0001. (D, E) Transwell migration assay and Transwell invasion assay were performed using HCT116 and RKO cells. (F, G) An in vitro Matrigel tube formation assay was performed to evaluate the angiogenic ability of human umbilical vein endothelial cells (HUVECs). The representative micrographs are shown at 200× magnification. (H, I) Representative images and bar graphs of liver and lung metastases with circFAM169A-knocked down HCT116 cells (H) and circFAM169A-overexpressing SW480 cells (I) in a nude mouse metastatic tumor model.