Research Paper Volume 15, Issue 16 pp 8315—8324
miR-100-5p activation of the autophagy response through inhibiting the mTOR pathway and suppression of cerebral infarction progression in mice
Figure 4. The in vitro BV-2 cell experiment. Overexpression of miR-100-5p (OE) decreased the protein expressions of phosphorylated mTOR and total mTOR, while increasing the expressions of autophagy-related proteins Beclin. (A) Representative Western blot images. (B) Column comparison of the relative expression of proteins. LC-3II, and ATG-7 compared with the empty vector group (NC) and miR-100-5p inhibitor group (KD). mTOR, mammalian target of the rapamycin; LC-3II, microtubule-associated protein light chain 3II; ATG-7, autophagy-related gene 7; NC, negative control; KD, knock down; OE, overexpression. **P<0.01.