Research Paper Volume 15, Issue 16 pp 8298—8314

Figure 5. The effects of Gastrodin intervention and PAK2 inhibition on the PI3K/AKT/GLUT4 pathway in the primary hippocampal neurons during hyperglycemia. (A) CCK-8 analysis of hippocampal neurons exposed to 50 mM glucose in the NC, DM, 2 μM, 5 μM, 10 μM and 20 μM of FRAX597 intervention groups. Western blot analysis of PAK2 (B), PI3K (C), p-AKT (D) and GLUT4 (E) protein expression in the primary hippocampal neurons of the NC, DM, DM+G and DM+I groups. Bar graphs represented optical density of these factors normalized with β-actin. *p < 0.05, **p < 0.01 and ***p < 0.001.