Figure 2. Characteristics of the Ovx/Sham and OA/YA phosphoproteomes. Proteome Discoverer (v2.4) was used for database search and identification of phosphopeptides and proteins. Venn diagram of identified phosphopeptides unique to each group and common to both groups in (A) Ovx/Sham and (B) OA/YA datasets. Prevalence of phosphorylation on amino acid residues serine (S), threonine (T), and tyrosine (Y) in (C) Ovx/Sham and (D) OA/YA datasets. Volcano plots of differentially regulated phosphopeptides (p < 0.05 and 1.4-fold change) in (E) Ovx/Sham and (F) OA/YA mice.