Research Paper Volume 15, Issue 15 pp 7362—7380

Figure 1. Schematic of experimental design created with https://www.biorender.com and mouse characteristics. (A) 6 mo C57BL/6J female mice were assigned to a Sham or Ovx group and underwent their respective surgeries. YA and OA mice were 4 mo and 24 mo, respectively. The left leg of anesthetized mice was subjected to in vivo contractions and then tibialis anterior muscles were immediately dissected. Frozen TA muscles underwent peptide extraction with trypsin digestion and TiO₂ phosphopeptide enrichment. nLC-MS/MS was performed on the Orbitrap Fusion Tribrid mass spectrometer for label-free phosphoproteomic analysis. (B) Body mass of all four groups of female mice measured before the terminal contraction experiment. Data was analyzed by a one-way ANOVA with Tukey’s multiple comparison test (p < 0.001); n = 3–4/group. (C) Uterine mass of Ovx and Sham mice dissected and weighed after the terminal contraction experiment. Data was analyzed by a pooled t-test (Ovx vs. Sham, p = 0.016); n = 4/group. Values represents mean ± SD. ^*p < 0.05, ^***p < 0.001, ^****p < 0.0001.