Research Paper Volume 15, Issue 16 pp 8113—8136

Figure 2. Identification of three distinct pyroptosis phenotypes in gastric cancer. (A, B) Integration of four gastric cancer cohorts (GSE15459, GSE62254, GSE84437 and TCGA-STAD) into one meta-cohort and removal of batch effects. (C) Heat map showing the optimal clustering by k = 3 based on prognostic pyroptosis-related genes in the meta-cohort. (D) The consensus cumulative distribution function (CDF) for the cumulative distributive function of the consensus matrix for k = 2–9 identified by different colors. (E) The CDF plots of consensus clustering matrix for k = 2–9. (F) The t-SNE plots of the mRNA expression profiles of prognostic pyroptosis-related genes showing the three distinct subtypes indicated by different colors. (G) Kaplan–Meier survival curves for the three pyroptosis subtypes based on the 1275 gastric cancer specimens in the meta-cohort (log-rank test). (H) Heatmap showing the GSVA score of the 50 hallmark pathways in three pyroptosis phenotypes.