Research Paper Volume 15, Issue 16 pp 8061—8089

Figure 4. Neuroprotective effects of LM-021 and NC009-1 on A53T-8 SNCA-GFP BE(2)-M17 cells. (A) Experimental flow chart. Cells were plated with the presence of retinoic acid (RA, 5 μM) on day 1. On day 2, cells were treated with LM-021 or NC009-1 (10 μM) for 8 h, followed by inducing SNCA-GFP expression (Dox, 5 μg/ml), and further added with α-synuclein fibril (0.1 μM). On day 7, cellular LDH, caspase-1, ROS and GSH/GSSG were assessed. (B) LDH activity, (C) caspase-1 activity, (D) ROS production and (E) GSH/GSSG ratio of A53T-8 SNCA-GFP BE(2)-M17 cells with or without induced SNCA-GFP expression, preformed fibril addition, or LM-021/NC009-1 treatment (n = 3). P values: with vs. without doxycycline induction (^#: P < 0.05, ^##: P < 0.01, ^###: P < 0.001), with vs. without addition of fibrils (^&&: P < 0.01), or with vs. without compound treatment (*: P < 0.05, **: P < 0.01, ***: P < 0.001).