Research Paper Volume 15, Issue 15 pp 7794—7810

Figure 5. Hmgcs2 promotes macrophage M2 polarization by activating Src/PI3K/AKT pathway. (A) Relative expression of Hmgcs2 and Src/PI3K/AKT pathway-related proteins in cells from each group. (B) Statistical results of relative expression of Hmgcs2 and Src/PI3K/Akt pathway-related proteins of cells in each group. (C) The mRNA relative expression of TNF-α of macrophages in each group. (D) The mRNA relative expression of IFN-β of macrophages in each group. (E) The mRNA relative expression of IL-6 of macrophages in each group. (F) The mRNA relative expression of IL-10 of macrophages in each group. (G) Concentrations of TNF-α for macrophages in each group (pg/ml). (H) The concentration of IFN-β in the macrophages of each group (pg/ml). (I) The concentration of IL-6 in macrophages from each group (pg/ml). (J) The concentration of IL-10 in macrophages from each group (pg/ml). (K) The flow cytometry of the macrophage polarization in distinct groups. Abbreviations: NC: control group; LPS: LPS induced M1 type macrophages; LPS+ empty: M1 macrophage group transfected with empty plasmid; LPS+Hmgcs2: Hmgcs2 over-expressing M1 macrophage group; LPS+Hmgcs2+PP1: M1 macrophages with over-expression of Hmgcs2 based on PP1 administration; LPS+IL-4: LPS+IL-4 induced M2 type macrophages. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001: vs. NC group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001: vs. LPS + HMGCS2 group.