Research Paper Volume 15, Issue 15 pp 7689—7708

Figure 9. Effect of BXD on PI3K/AKT pathway and inflammatory factors in LPS-induced neuroinflammation. (A–F) Expression levels of PI3K, AKT, P-AKT, MMP9, NF-κB and TNF-α proteins were measured by Western blot, β-actin served as an internal Control. (G) The mRNA expression levels of TNF-α was detected by RT-PCR. (H) The secretion of IL-1β was detected by ELISA. (I) The mRNA expression levels of IL-1β was detected by RT-PCR. (J) The secretion of NO was detected by ELISA. Data represent the mean ± SEM (n = 3 − 6). ^****P < 0.0001, ^***P < 0.001, ^**P < 0.01, ^*P < 0.05. Compared with the LPS group.