Research Paper Volume 15, Issue 15 pp 7673—7688

Figure 4. The deletion of Nrf2 contributed to ferroptosis in HK-2 cells. The Nrf2 protein expression was analyzed by western blotting and semi-quantitative analysis in renal tissues (A, B). The mRNA expression of Nrf2 (C). The expression level (D) as well as semi-quantitative analysis (E) of Nrf2 (D). The mRNA expression of Nrf2 (F). The expression levels as well as semi-quantitative analysis of ferroptosis related protein (G, H). The changes in iron and MDA (I, J) were measured. ^*P < 0.05, ^**P < 0.01 vs. normal group, ^#P < 0.05, ^##P < 0.01 vs. model group.